Innovation that transforms lives

For years, in vitro fertilisation improved almost everything: embryo selection, culture media, the efficiency of each cycle. Almost everything but one thing. When the egg was of poor quality, medicine had no answer, and the conversation always ended in the same place: egg donation.

IVF MORE® came out of that conversation. It is a technique created by Dr. Shoukhrat Mitalipov and developed alongside Ingenes, and its purpose is to restore the egg’s cellular function before fertilisation, without touching its genetic material.

• +40patients treated with the technique
• +19babies born through this technology
• 100%yours: the genetic material is not modified
• 1mature egg is enough to try

The origin of IVF MORE®

IVF MORE® is not just an in vitro fertilisation procedure. It is the result of years of research in reproductive biotechnology, molecular biology and advanced micromanipulation. The patent is in progress, and the results are in the process of being published in high-impact scientific journals.

In collaboration with Dr. Shoukhrat Mitalipov

The science behind the technique

IVF MORE® combines four technologies that work at the cellular and metabolic level. None of the four solves the problem on its own; the result comes from applying them in order to the same egg.

1. 01

   Oocyte metabolic diagnosis

   The mitochondria, the cell's powerhouses, are analysed for the ATP, NADPH and other key molecules they supply.⁴ That gives the egg's energy status. If there is a deficit, restoration follows.

2. 02

   Restoration with advanced micromanipulation

   Through intracytoplasmic infusion, mitochondrial metabolic factors are incorporated,³ and the cytoskeleton is allowed to reorganise, which is what aligns the internal fibres for correct cell division.

3. 03

   Fertilisation with optimal selection

   Microfluidics and hyaluronan adhesion select the most competent sperm. The PICSI technique ensures that only those with the highest potential fertilise the restored eggs.

4. 04

   Embryo culture in magnetic fields

   Exposure to magnetic fields reduces oxidative stress, optimises gene expression and improves cellular organisation.² Development is more stable and favours viable blastocysts.

Development of the technique

Egg bank

The process begins by retrieving the patient’s eggs. To increase the number, we apply different hormonal stimulation protocols.

If we obtain more than six eggs and they are of good quality, a single session is enough. If they are few, or look dark and granular, we suggest a second stimulation to gather enough quality eggs before applying the technique. The goal is to freeze them in less than six months.

Even if a patient produces very few eggs we can proceed: one mature egg is enough to attempt the treatment. And that is where the first sorting happens, because not everything retrieved at the puncture can be fertilised.

• 

  Germinal vesicle

• 

  Metaphase I

• 

  Metaphase II

• Immature
• Mature

A mature egg looks like this

• Translucent
• Soft shell
• No granules
• A single polar body

Metabolic diagnosis

We have identified factors that let us detect the metabolic state of each egg and reach a diagnosis. Some, such as Annexin V and other Krebs cycle markers, are decisive for its metabolic development. The profile that comes out tells us whether the egg has enough metabolites and energy to divide correctly.⁵

That diagnosis allows quality to be assessed with more precision, and the effort to be concentrated on the eggs with the most favourable metabolic conditions.

Egg restoration

We improve the egg with a patented method: an intracytoplasmic transfusion into low-quality oocytes with growth factors and essential energy components derived from the patient’s own cells. That gives the egg greater chances of fertilisation and reduces the risk of producing an embryo with genetic abnormalities.

The method uses biomolecular technology and nanoplatelet-based digital PCR. Together, it combines cutting-edge technology with components personalised to each case.

In vitro fertilisation

We use PICSI, Physiological Intracytoplasmic Sperm Injection, to fertilise the eggs. What matters there is selecting the most capable sperm.

There is an alternative in which sperm travel along a channel using a microfluidics technique. The best-quality ones manage to cross it from one end to the other. The selected sperm bind to hyaluronan, an essential component of the zona pellucida, the egg’s outer layer. By simulating the natural process we can filter out those with the greatest capacity to compete and advance.

Integrating PICSI with microfluidics improves the precision of that choice, and with it fertilisation rates and embryo quality.

1. Sample

   Everything goes in, capable and slow alike.

2. Microfluidic channel

   Only the better swimmers get through.

3. Hyaluronan binding

   Mature sperm bind, and are selected.

Culture in magnetic fields

We found that leaving restored eggs in magnetic fields produces an intracytoplasmic movement of solutes that allows better organisation of the cytoskeleton. Once fertilised, we increase the potential of the fields so the egg spends less energy and divides correctly.

Put another way: magnetic fields have significantly increased the possibility of producing viable embryos.²

Embryo transfer to the uterus

With viable embryos, transfer is the step that closes the process. We prepare the recipient’s endometrium with hormonal treatments, oestrogen and progesterone, to thicken and stabilise the uterine lining and make it more receptive.

In some cases we use regenerative medicine techniques to increase implantation potential. Combining careful endometrial preparation with those techniques creates the most favourable conditions for a pregnancy to hold.

Mission, vision and values

• Mission

  Innovation to enhance health

  Our pursuit of research and development leads to therapies and treatments with the power to improve our patients' health through regenerative medicine, and to let them live fuller lives.

• Vision

  Research grounded in what matters most: your family

  It is not just about science. It is about translating that science into real benefits for people, knowing that it makes a genuine difference.

• Values

  Commitment to excellence

  Excellence and expertise

  The highest standards in everything we do, from research to patient care.

  Practicality

  A professional but accessible approach, so the innovations stay within reach.

  Integrity

  Ethical standards are the foundation of our research.

  Commitment

  Our dedication to advancing healthcare is unwavering.

  Innovation

  We are constantly looking for new ways to improve health outcomes.

The future of fertility

Putting IVF MORE® into practice meant adapting laboratories: latest-generation micromanipulation, high-resolution microscopy and devices to generate controlled magnetic fields.

Thanks to that work, there is now an alternative that preserves the patient’s genetic material and gives her the possibility of having a baby with her own eggs, even in cases where it was not possible before.

IVF MORE® is not just a treatment. It is the evolution of assisted reproduction, and an innovation grounded not only in technology but in the conviction that every woman deserves a real opportunity to become a mother with her own biological resources.