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IVF MORE® process

How IVF MORE® works, step by step

The full IVF MORE® journey, from the first consultation to embryo transfer: what happens at each stage, what the laboratory does, and which treatments complement it.

An Ingenes embryologist at the micromanipulator: on screen, the ICSI needle entering an egg held by the holding pipette

The goal of IVF MORE® is to restore poor-quality eggs and give them a new chance to become viable embryos. The full treatment takes around three months and is carried out in the specialised Ingenes laboratory in Mexico City.

A single mature egg is enough to move forward.

An abundant harvest is not the requirement. What the technique needs is at least one mature egg to work on.

  • 3 monthsis how long the full treatment takes, consultation to transfer
  • 1mature egg is enough for the technique to be applied
  • 7stages in your journey as a patient
  • 12steps inside the Ingenes laboratory

Your path, step by step

  1. Consultation

    Initial consultation

    We review your case and your history to determine whether IVF MORE® is the right option for you.

  2. At the clinic

    Ovarian stimulation and retrieval

    The ovaries are stimulated to obtain eggs. On retrieval they are vitrified alongside the sperm sample, where applicable.

  3. In the laboratory

    Metabolic diagnosis of the egg

    Each egg is measured on its own: ATP production, mitochondrial function, apoptosis factors and spindle structure.4,5

  4. In the laboratory

    IVF MORE® treatment

    Energy and mitochondrial factors are infused to reinforce the deficiencies of the egg.1 If donor cytoplasm is needed, your nucleus is still never touched.

  5. In the laboratory

    In vitro fertilisation via ICSI

    The best sperm are selected, with techniques such as PICSI, and the restored egg is fertilised.

  6. In the laboratory

    Culture in magnetic fields

    Embryos grow in magnetic fields that support the cytoskeleton and favour accurate cell division.2

  7. At the clinic

    Embryo transfer

    The endometrium is prepared and one or more restored embryos are transferred.

The full process, inside the laboratory

The seven steps above are your journey. Inside the laboratory, each one breaks down into finer operations: separating the egg from the granulosa cells, cryopreservation, aspirating the cytoplasm, infusing metabolites, culture in magnetic fields and the assessment of embryo viability.

A gloved hand holding a culture dish lit from below under the microscope, in the Ingenes laboratory
Ingenes laboratory, Mexico City.

  1. Separation and cryopreservation

    The egg is isolated from the granulosa cells around it and vitrified alongside the sperm sample.

  2. Metabolic diagnosis

    A minimal cytoplasm sample is drawn and each egg's energy competence is measured on its own.

  3. Restoration and ICSI

    The factors that this particular egg needs are infused, and it is fertilised by intracytoplasmic sperm injection.

  4. Culture and assessment

    The embryo develops in magnetic fields and its viability is assessed before transfer.

Circular infographic of the IVF MORE® process in twelve steps, from egg retrieval to the transfer of the restored embryo, taking in metabolic diagnosis, cytoplasm aspiration, infusion of metabolites and culture in magnetic fields
The egg's full route through the laboratory. At the end of the circuit, the comparison that sums the technique up: 27% genetic abnormalities with IVF MORE® against 82% without restoration. View full size.

What happens during restoration

This is the stage that defines the technique. The egg’s cytoplasm is worked on to give back the energy and machinery it lost, while the nucleus stays untouched.

Restoration is achieved by infusing energy and mitochondrial factors into the egg, reinforcing the weakened cellular functions.1

  • How to read the diagram
  • The egg's own energy machinery, weakened
  • Growth factors and energy components infused
  • The patient's genetic material
  • The sperm's contribution

  1. 01

    Cytoplasm biopsy

    A micropipette draws a minimal sample of cytoplasm. That sample is what gets analysed to measure ATP production, mitochondrial function and apoptosis markers, and to decide what this particular egg needs.

  2. 02

    Factor infusion and ICSI fertilisation

    Through the same route, growth factors and energy components are infused to reinforce the weakened cell functions, and the selected sperm is introduced. The patient's spindle and chromosomes are never touched.

  3. 03

    Restored egg, untouched nucleus

    The cytoplasm can once again sustain cell division, and the two pronuclei form normally. If donor cytoplasm was needed, there is still no foreign genetic material: the embryo's DNA is yours and your partner's or sperm donor's.

A reconstruction of the IVF MORE® clinical schematic. Restoration works on the cytoplasm, which is where the cell keeps its energy. The nucleus, where your genetic information lives, stays out of the procedure.

In some cases cytoplasm from donor eggs may be needed, but that does not modify the genetic load of the patient’s egg: the genetic material remains 100% yours. Restoration opens the possibility of reducing the genetic alterations tied to energy deficiencies.

Illustration of an embryo at its first cell division, with the two nuclei visible inside the zona pellucida
The first cell division. This is the moment where it shows whether the cytoplasm had what it takes: how the chromosomes are shared out depends on energy and cytoskeleton organisation, not on the nucleus.

Frequently asked questions

How long does the full treatment take?

The complete treatment takes approximately 3 months.

When is it advisable to accumulate more eggs?

Even with a single mature egg we can apply IVF MORE®. If there are no viable eggs, a second stimulation may be considered.

Where is the procedure carried out?

Retrieval and restoration take place in the specialised Ingenes laboratory in Mexico City. If your eggs are frozen at another clinic or in another country, their transfer can be arranged.

What are the limitations of the technique?

IVF MORE® requires at least one mature egg. If none is produced, even with stimulation, the technique cannot be applied.

In some cases, even when eggs are available, they may not be viable or may not respond adequately to restoration. When that happens, egg donation may be the most effective alternative for achieving a pregnancy.

Complementary treatments

IVF MORE® acts on the egg. But a pregnancy also depends on how many eggs can be obtained, on the state of the endometrium that will receive the embryo, and on what genetic information that embryo carries. There is a treatment for each of those fronts.

Before: ovarian response

OVAGEN®, for more functional eggs

In patients with low ovarian reserve or poor response, the first challenge for IVF MORE® is obtaining enough functional eggs. OVAGEN® is a regenerative medicine treatment designed to optimise ovarian response by:

  • Increasing ovarian vascularisation, improving the supply of oxygen and nutrients.
  • Stimulating follicular development, encouraging the production of more eggs.
  • Reactivating ovarian function in women with low reserve or poor response to previous treatments.

The procedure applies growth factors obtained from the patient's own blood plasma (PRP) directly, alongside other bio-stimulants that promote ovarian regeneration.6

How it complements: it widens the possibilities for patients who, given their condition, would produce few or no oocytes. By improving both quantity and quality, it increases the number of eggs available for restoration.

After: the endometrium

ENDOGEN®, for endometrial receptivity

The endometrium, the inner lining of the uterus, is decisive in implantation. For a restored embryo to implant, the endometrium needs adequate thickness, vascularisation and receptivity. A thin endometrium can stop even a genetically normal embryo from implanting.

ENDOGEN® uses growth factors and, in some cases, mesenchymal stem cells to improve endometrial quality, particularly in patients with:

  • A thin endometrium, under 7 mm, or documented low receptivity.
  • Recurrent miscarriage from implantation failure.
  • Conditions that alter the uterine environment, such as Asherman's syndrome.7

Platelet-rich plasma is drawn from the patient, enriched with endometrial growth factors, and applied directly to the endometrium in a minimally invasive procedure before transfer.

How it complements: once the eggs are restored and viable embryos obtained, ENDOGEN® acts on the other critical component of pregnancy, the uterus.

During: assessing the embryo

Non-invasive PGT-A, genetic evaluation without biopsy

Instead of extracting cells from the embryo, as a conventional biopsy does, the DNA the embryo itself releases into the culture medium is analysed.

  • Less stress on the embryo: its outer layer is not perforated.
  • Detection of chromosomal anomalies such as Down, Turner or Klinefelter syndrome.
  • Fast results without compromising the embryo's viability.

Limitations: some embryos do not release enough genetic material into the medium, and a traditional PGT-A diagnosis would then require a biopsy. For IVF MORE®, biopsying the embryo is not recommended.

How it complements: IVF MORE® reduces genetic alterations in embryos; non-invasive PGT-A adds one more layer of verification before transfer.

If you already froze

Compatibility with previously frozen eggs

If you froze eggs in the past, IVF MORE® can help optimise their chances. Once your eggs are in the laboratory, whether retrieved in Mexico City or transferred there, a metabolic diagnosis assesses their quality and restoration is applied before fertilisation.

It is particularly worth considering if you froze after 35, if your eggs show signs of poor quality when thawed, or if you have had failed fertilisation attempts with them.

Are they at another clinic or in another country? Ingenes works with companies specialising in the transport of biological material and can arrange the transfer to Mexico in conditions suitable for restoration.

Each of these treatments complements the IVF MORE® methodology to build a personalised plan. If you are unsure which applies to your case, our medical team can assess it with you.

Scientific sources9
  1. Woods D.C., Tilly J.L. (2015). Autologous Germline Mitochondrial Energy Transfer (AUGMENT) in Human Assisted Reproduction. Seminars in Reproductive Medicine.
  2. Zablotskii V., et al. (2016). How a High-Gradient Magnetic Field Could Affect Cell Life. Scientific Reports (Nature).
  3. Oregon Health & Science University. Meet Shoukhrat Mitalipov, Ph.D., Center for Embryonic Cell and Gene Therapy. OHSU.
  4. Zhu Q., Li Y., Ma J., Ma H., Liang X. (2023). Potential factors result in diminished ovarian reserve: a comprehensive review. Journal of Ovarian Research.
  5. Wang L., et al. (2021). Oxidative stress in oocyte aging and female reproduction. Journal of Cellular Physiology.
  6. Cakiroglu Y., et al. (2022). Ovarian reserve parameters and IVF outcomes in 510 women with poor ovarian response treated with intraovarian injection of autologous platelet-rich plasma. Aging.
  7. Hernández-Melchor D., et al. (2024). Improvement of endometrial thickness and in vitro fertilization outcomes in patients with Asherman's refractory endometrium using autologous mesenchymal stem cells from the stromal vascular fraction. American Journal of Translational Research.
  8. Hernández-Melchor D., et al. (2023). Personalized regenerative strategies and molecular diagnosis for in vitro fertilization success. Oxford Medical Case Reports.
  9. Society for Assisted Reproductive Technology (2022). Live births per intended egg retrieval, national summary report. SART CORS.

Next steps

Take the first step toward your baby with IVF MORE®

Our team is ready to support you every step of the way.

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