Step by Step

By addressing key aspects of egg health and cellular metabolism, IVF MORE® offers a scientifically advanced alternative for patients who would otherwise require donor eggs.

Where do I start?

A clear step-by-step guide to understanding your journey with IVF MORE®

Initial consultation

Your first medical appointment to review your case, learn about your history, and determine whether IVF MORE® is the right option for you.

Ovarian stimulation and retrieval

The ovary is stimulated to obtain mature eggs. At the same time, a semen sample is collected (if applicable).

Separation and cryopreservation

Eggs are separated from granulosa cells, and everything is frozen: eggs, granulosa, and semen.

Thawing

When everything is ready to proceed, the previously vitrified material is thawed.

Metabolic diagnosis

The metabolism of the egg’s cytoplasm is analyzed to evaluate its energy capacity and determine the type of restoration needed.

Sperm selection and ICSI

The best sperm are selected using techniques like PICSI or microfluidics and injected directly into the egg.

Cytoplasmic aspiration and infusion

A small portion of the egg’s cytoplasm is aspirated. → If the egg’s metabolism is deficient, cytoplasm from a donor egg will be used, without altering the patient’s genetics.

Restoration and fertilization

An infusion of mitochondrial, energy, and growth factors is performed.
Simultaneously, the sperm is injected (ICSI).

Magnetic field culture

Embryos are cultured in an optimized environment that promotes healthy cell division and reduces chromosomal errors.

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Embryo transfer

Viable embryos are transferred in a deferred cycle, after preparing the uterus with hormonal support or ENDOGEN®, if needed.

1. Ovarian Stimulation and Egg Retrieval

The process begins with ovarian stimulation to induce egg production. This protocol is personalized for each patient and follows an antagonist cycle with FSH, menotropin, and, in some cases, letrozole to optimize ovarian response.

If at least one viable egg is obtained, the process can proceed with a single stimulation.
If the eggs are of low quality (dark color, granules, etc.), a second stimulation is recommended to increase the number of eggs before restoration.

Once collected, the eggs and granulosa (a group of cells that surround the oocytes) are separated so they can be frozen along with:
-The semen sample (if there is a partner)
-The granulosa cells, which are essential for the restoration process

2. Egg Metabolic Diagnosis

After thawing the eggs, the sperm (if applicable), and the granulosa, we perform a molecular evaluation of the oocyte metabolism and cytoplasm using tools such as digital PCR and metabolite analysis.

What is the metabolic diagnosis of an egg?

This diagnostic process allows us to:

Identify apoptosis markers (programmed cell death).
Evaluate ATP and NADPH production, which are crucial in the Krebs cycle.
Determine if the egg has enough energy to divide properly.

If an egg presents energy deficits, its restoration is customized with growth factors and mitochondrial precursors.

3. Optimized Sperm Selection

To ensure effective fertilization, we use:

Microfluidics for sperm selection → Only the most motile sperm advance through a channel, eliminating those with morphological defects.
Hyaluronan binding → Mimicking natural selection, ensuring that the selected sperm are the most competent.
PICSI technique → Physiological Intracytoplasmic Sperm Injection using sperm with optimal characteristics.

This approach maximizes the probability of successful fertilization and the formation of high-quality embryos.

4. Oocyte Restoration: The Core of IVF MORE®

IVF MORE® oocyte restoration is based on advanced micromanipulation and regenerative biotechnology to revitalize eggs before fertilization. To do this technique, we need different labs and equipment than a regular IVF treatment given the procedures are more specialized.

The procedure includes:

Aspiration of cytoplasm for the infusion of energy and growth factors.
Intracytoplasmic infusion of energy and growth factors.
Use of granulosa cells to supply key metabolites.
Cytoskeletal restructuring to optimize cell division.

By restoring the egg's metabolism, IVF MORE® drastically reduces the risk of chromosomal abnormalities and increases the chances of obtaining a viable embryo.

In some cases, after the metabolic diagnosis, it is determined that the patient’s own egg does not have sufficient energy capacity to divide properly. When this happens, we may use the cytoplasm of a donor egg as an additional source of metabolites and mitochondrial factors needed for the process. The patient’s genetic material is preserved at all times; only metabolic factors from the donor egg’s cytoplasm are injected to support cell division.

How does oocyte restoration help prevent genetic errors?

Proper cell division depends on two key components within the egg:

The cytoskeleton, which acts like an internal scaffold that organizes cellular components.
The meiotic spindle (or acromosomal spindle), a structure formed from the cytoskeleton that aligns and separates chromosomes correctly during fertilization.

When an egg has poor metabolic quality (due to advanced age, endometriosis, PCOS, etc.), it does not produce enough energy. This leads to disorganization of the cytoskeleton and the meiotic spindle.

What are the consequences?
Incorrect cell division, in which chromosomes are not distributed evenly. This is called nondisjunction, and it can lead to genetic abnormalities such as:

Trisomies (e.g., Down syndrome)
Monosomies (e.g., Turner syndrome)
Non-viable embryos

With IVF MORE®, by restoring the oocyte cytoplasm with energy and mitochondrial factors:

The cytoskeleton is reorganized
The meiotic spindle is stabilized
And precise, symmetrical genetic division is enabled

This significantly reduces the risk of chromosomal errors and increases the likelihood of obtaining viable embryos.

5. Magnetic Field Culture: Optimized Embryo Development

The obtained embryos are cultured in a specialized magnetic field environment, which has been shown to:

Reduce oxidative stress and cellular inflammation.
Optimize embryonic cytoskeletal organization.
Facilitate proper gene expression and cell division.

This environment promotes the formation of viable blastocysts with a lower rate of genetic errors.

One of the most prestigious journals worldwide published how magnetic fields can enhance embryonic development and cell division.

Magnetic Fields – Nature: https://www.nature.com/articles/srep37407

Desregulación metabólica en los ovocitos de pacientes con baja reserva ovárica: Zhu, Q., Li, Y., Ma, J., Ma, H., & Liang, X. (2023). Potential factors result in diminished ovarian reserve: a comprehensive review. Journal of Ovarian Research, 16(1). https://doi.org/10.1186/s13048-023-01296-x

Autologous Germline Mitochondria Energy Transfer : https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5545901/pdf/nihms880936.pdf

6. Embryo Transfer and Regenerative Medicine

Viable embryos are transferred in a deferred cycle, allowing the uterus to be optimally prepared.

Hormonal treatments (estrogen and progesterone) are used to thicken the endometrium.
In cases of low endometrial receptivity, Endogen®—a combination of platelet-rich plasma and growth factors—is recommended to improve implantation.
Mesenchymal stem cells can also be used in patients with extremely thin endometrium.

This comprehensive approach maximizes implantation rates and the chances of a successful pregnancy.

Additional Treatments

IVF MORE® is not only a groundbreaking advancement in egg restoration, but it also integrates with other innovative treatments that optimize egg quality, endometrial receptivity, and embryo genetic evaluation.

OVAGEN®: Enhancing Egg Quality

In patients with low ovarian quality, one of the main challenges is obtaining a sufficient number of functional oocytes. OVAGEN® is a regenerative medicine treatment aimed at optimizing ovarian response by stimulating the body’s own repair mechanisms. This procedure is designed to:

✔ Increase ovarian vascularization, improving the delivery of oxygen and essential nutrients.
✔ Stimulate follicular development, encouraging the production of a higher number of oocytes.
✔ Reactivate ovarian function in women with diminished ovarian reserve or poor response to previous treatments.

The procedure involves the direct application of growth factors and regenerative proteins derived from the patient’s own blood plasma (PRP), along with other bio-stimulants that promote ovarian regeneration.

Multiple studies have shown that this technique can help restore ovarian activity in women who previously failed to produce oocytes, thereby improving their response to ovarian stimulation.

How does it complement IVF MORE®?

OVAGEN® expands the possibilities for patients who, due to their condition, would produce few or no oocytes in a conventional cycle. By improving both the quantity and quality of oocytes, this treatment increases the number of oocytes available for restoration through the IVF MORE® technique, significantly enhancing their chances of success.

ENDOGEN®: Optimizing Endometrial Receptivity

The endometrium—the inner lining of the uterus—plays a critical role in embryo implantation. For an embryo restored through IVF MORE® to successfully implant, the endometrium must be in optimal condition in terms of thickness, vascularization, and cellular receptivity.

ENDOGEN® is a regenerative medicine treatment that uses growth factors and, in some cases, mesenchymal stem cells to improve endometrial quality, especially in patients with:

✔ Thin endometrium (less than 7 mm) or documented low receptivity
✔ Recurrent implantation failure or early pregnancy loss
✔ Conditions that alter the uterine environment, such as endometriosis or Asherman’s syndrome

Low receptivity = Thin endometrium
Low endometrial receptivity is often associated with a thin lining or poor vascular development. A thin endometrium may prevent even a genetically healthy embryo from properly attaching and developing.

How does ENDOGEN® work?

Platelet-rich plasma (PRP) is extracted from the patient’s own blood.
This PRP is enriched with endometrial growth factors and, when needed, mesenchymal stem cells.
The solution is applied directly to the endometrium in a minimally invasive procedure prior to embryo transfer.

How does it complement IVF MORE®?

Once oocytes have been restored and viable embryos created with IVF MORE®, ENDOGEN® supports the other essential component of pregnancy: the uterus. By improving the endometrial environment, it significantly increases the implantation rate and the likelihood of a successful pregnancy.

Non-Invasive Genetic Screening:

PGT-A Without Biopsy

One of the most significant advances in fertility treatment is the ability to analyze the genetic health of embryos without requiring a biopsy.

How Does It Work?
Instead of extracting cells from the embryo, as in conventional biopsy-based testing, we use an advanced technique that analyzes cell-free DNA released by the embryo into the culture medium.

Benefits of Non-Invasive PGT-A:

Less stress on the embryo: No need to puncture the embryo’s outer layer.
Highly accurate detection of chromosomal abnormalities: Identifying conditions like Down syndrome, Turner syndrome, Klinefelter syndrome, and others.
Fast and reliable results: Without compromising embryo viability.

Limitations
Sometimes embryos do not release enough genetic material into the culture medium, and a biopsy may be needed to obtain information. However, in IVF MORE®, embryo biopsy is not recommended due to the prior micromanipulation involved in the restoration process. If genetic information cannot be obtained through non-invasive PGT-A, it is advised not to perform a biopsy.

Live Births Per intended Egg Retrieval SART : https://sartcorsonline.com/CSR/PublicSnapshotReport?/ClinicPKID=0&reportingYear=2022

How Does It Complement MORE®?

IVF MORE® reduces genetic abnormalities in embryos. However, for additional safety, we offer Non-Invasive PGT-A as an extra step in embryo evaluation.

Compatibility with Previously Frozen Eggs

If you previously froze eggs with the hope of using them in the future, IVF MORE® can help optimize their chances of success.

How Does It Work?

Your frozen eggs are transported to our Ingenes Mexico laboratories.
We conduct a metabolic diagnosis to evaluate their quality.
IVF MORE® is applied to restore the eggs before fertilization.

When Is It Recommended to Restore Frozen Eggs?

If you froze your eggs after age 35.
If your eggs show signs of poor quality upon thawing.
If you’ve had previous failed fertilization attempts with your frozen eggs.

Are Your Eggs Stored in Another Clinic or Country?

No problem! At Ingenes, we work with specialized biological material transport companies and can coordinate the safe transfer of your eggs to Mexico, ensuring they arrive in optimal conditions for restoration.

A Personalized Treatment for Every Patient

Whether you need to Optimize your egg quality with OVAGEN®, improve endometrial receptivity with ENDOGEN®, or perform non-invasive genetic testing with PGT-A, each of these treatments complements the MORE® methodology to provide you with the best chance of achieving a healthy pregnancy.

Take the Next Step Toward Growing Your Family

We’re here to support you at every stage of the journey.